Coding

Part:BBa_K2924027:Design

Designed by: Andreas Nakielski, Melanie Sbielut   Group: iGEM19_Duesseldorf   (2019-10-12)


α-s2-casein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 667
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 97


Design Notes

The sequence is codon-optimized for expression in E. coli

Source

The DNA sequence was acquired by reverse-translating the protein sequence of a-s2-casein.

References